Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging.

TitleUltra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging.
Publication TypeJournal Article
Year of Publication2012
AuthorsPodgorski K, Terpetschnig E, Klochko OP, Obukhova OM, Haas K
JournalPLoS One
Volume7
Issue12
Paginatione51980
Date Published2012
ISSN1932-6203
KeywordsAnimals, Cyclobutanes, Fluorescent Dyes, Microscopy, Fluorescence, Multiphoton, Nerve Tissue Proteins, Neurons, Phenols, Photons, Rotaxanes, Synapses, Xenopus laevis
Abstract

Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/23251670
DOI10.1371/journal.pone.0051980
Alternate JournalPLoS ONE
PubMed ID23251670
PubMed Central IDPMC3522634
Grant List / / Canadian Institutes of Health Research / Canada